Introduction: The effect of emotion regulation on life difficulties has been a focus of attention for researchers and scientists. The aim of this investigation was to examine the relationships among affect regulation and worry with mental health and quality of life.Method: The sample consisted of 193 students from Imam Khomeini University who were selected by a categorical randomized method. The General Health Questionnaire, the Difficulties in Emotion Regulation Scale, the Short Form (36) Health Survey and the Worry Questionnaire, were used to measure variables. Data were analyzed using correlation coefficient and regression analysis.Results: Significant relationships were observed between affect deregulation and worry about quality of life and mental health. Among affect regulation components, acceptance was positively related and clarity was negatively related with quality of life and mental health. Significant correlations were observed between worry and all components (except body-related components) of mental health and quality of life. The results of a regression analysis showed that worry, acceptance, and clarity predict variability in mental health and life quality.Conclusion: Emotion regulation can affect mental health and quality of life. Worry had a negative effect on quality of life and mental health.