Introduction: The purpose of this study was developing the healthy behavior to change the attitude of students in order to decrease their tendency towards substance abuse. .Method: This research was carried out by two methods of descriptive- analytic and quasi- experimental design. First, 60 students of Allameh Tabatabai University were selected randomly (of 4000 students) from the University dormitory. Then, half of the students received the developing healthy behavior training program. The efficacy of this program was evaluated by the scale of “attitude towards substance abuse”. The data was analysed by the analysis of covariance and MANOVA..Result: The result of this research indicated that healthy behavior training program develops the negative attitude towards substance abuse. Developing negative attitude was significant in cognitive, emotional, and behavioral tendency. Also, this program was most effective on the cognitive aspect of attitude..Conclusion: The result of the study indicating the effectiveness of healthy behavior training program in changing the attitude of students towards substance abuse is compatible with previous studies. Therefore the implication of this program in order to prevent substance abuse is recommended.